In-Vitro and In-Vivo studies of Combination patch of Diclofenac Sodium and Tizanidine Hydrochloride

Transdermal Patches of Combination Drugs Diclofenac sodium-Tizanidine Hydrochloride were prepared using solvent casting method. The Patches were evaluated for various Physicochemical Parameters including In-Vitro and In-Vivo Studies. In-vitro Permeation Studies were conducted using franz diffusion cell on the Rat abdominal skin. The Permeability Coefficient values and Flux were calculated. Kinetics for drug release were calculated by Zero, First, Higuchi Model and Koresmeyer-Peppas model indicate that release mechanism follows Fickian Diffusion as the value of release exponent is less than 0.5 ranging in between 0.0847-0.1690 for Koresmeyer-Peppas model. The percent of drug permeated in 12 h was found to be maximum 99.8± 0.01 and 98.98± 0.01 % from formulations FA2 and FA8 respectively. The two best formulations were subjected to In-Vivo studies on male albino wister rats by carragenan induced Paw edema method. The formulations were compared with marketed formulation. It was observed the on apply ANOVA no significant difference is found between the Marketed Nu-Patch and best formulations. It was found that formulation FA8 with DSMO as Plastisizer and Span 80 as Penetration Enhancher is the best formulation in all the Evaluation Parameters.