Antimicrobial (Bacteria, Fungi) Activity of Andrographis Paniculata and Detection of Andrographolide through TLC & HPLC Technique

Andrographis paniculata is annual herbaceous plant belonging to acanthaceae family it widely distributed on Asian countries. It’s height about 30-110cm, its flowers having a rose purple spots on their petals. It is having a dark green stem about 0.3-1.0 m in height and 2-6 nm in diameter.Antiheptotoxicactivity, Antimalarial activity, Antithrombogenic activity, Anti-inflammatory activity, Antisnakevenom activity, respiratory system benefits, and antifertility activity. These are the main pharmacological potential of Andrographis paniculata. In addition the allopathic medicines are not curing all the human diseases for Eg; Jaundice, cancer, paralysis, diabetics, and skin diseases etc., which are not curable by allopathic medicines. Hence the perception of medicine turned around towards the herbal plants. There are five cultures were tested in this project, among that three of them is bacteria, and the remaining two of that are fungi bacteria i) vibrio cholera ii) klebsiella pneumonia.iii) salmonella typhi. Fungi Candida albicans, cryptococci. All of these cultures are purchases from Tamilnadu Agricultural University (TNAU) Coimbatore. From this current investigation it was observed that the methanolic, petroleum ether and dichloromethane extract of Andrographis paniculata inhibited the growth of human pathogens 90%, 0% and 40% respectively. The above findings recommend the further investigations of Andrographis paniculata to evaluate their potential for use as antimicrobials to plant pathogens. The developed HPLC method can be utilized for the quantitative determination of andrographolide in Andrographis paniculata herb samples, extracts and dosage forms.HPTLC method also can utilized for quantitative determination of Andrographis paniculata herb samples, extracts and dosage forms. The complexity of the chemical composition of herbal extracts, quality of the herbal extracts can only be assured by the use of validated analytical methods for identification and quantification of the active ingredients. The HPLC and HPTLC methods for the quantitative estimation of andrographolide were validated with regard to their specificity, precision, accuracy and linearity. At the same retention time the leaf extract of Andrographis paniculata having a high amount of Andrographolide compare than their standard.