Gene Profiling of Toll Like Receptor in Indigenous Chicken and Japanese Quail

Toll like receptors (TLRS) are trans membrane proteins that detect the invading pathogens by binding conserved microbial derived molecules that induce signaling cascades to & pro inflammatory gene expression TLRs utilize leucine rich-motifs (LHRS) for ligand binding. The samples liver and spleen were collected from the bird’s indigenous chicken and Japanese quail from the moor market, Chennai. In the present study, DNA was isolated from the samples and DNA bands were separated in 0.8% gel. DNA was quantified, calculated and the DNA quantity was above 200ng. PCR was done for both TLR3 and TLR5 using outer primer as templates. Then the using outer primer, internal primers ip1, ip2, ip3 and ip4 were designed and used for both TLR3 and TLR5. And with the same, bulk was run for the internal primers to elute the amplified and purified DNA. The amplified DNA was extracted from 2% gel and then DNA was purified. It was sequenced using BLAST. Toll – like receptor (TLR) family are vital to immune function through the sensing of pathogenic agents and the initiation of immune response. And they are capable of sensing a wide range of microbes and quickly produce anti-microbial chemokine’s and cytokines. The TLR biology on various species modulates that system favorably affect a disease condition. So the TLR opens an exciting era of drug development. Avian TLR biology was different from mammals. There are opportunities to develop species –specific adjuvants and pathogen control strategies will be an impact on animal and human health. Gene profiling of toll-like receptors in indigenous chicken and Japanese quail was carried out in tamilnadu vetinery university, Chennai.