Effect of NAA and BAP on In-Vitro Micropropagation of Vitex negundo L. – A Significant Medicinal Plant
| Vol-4 | Issue-02 | February 2019 | Published Online: 20 February 2019 PDF ( 478 KB ) | ||
| DOI: https://doi.org/10.5281/zenodo.2580250 | ||
| Author(s) | ||
Arif Hussain Bhat
1;
Aparna Alia
2;
Ghulam Mustafa Rather
3;
Bharty Kumar
4
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1Department of Botany, Government M.V.M. Bhopal, Madhya Pradesh (India) 2Department of Botany, Rajeev Gandhi College, Shahpura, Bhopal, Madhya Pradesh (India) 3Department of Botany, Government M.V.M. Bhopal, Madhya Pradesh (India) 4Department of Botany, Government M.V.M. Bhopal, Madhya Pradesh (India) |
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| Abstract | ||
Vitex negundo has been used worldwide in pharmaceutical industry. Its natural vegetative propagation rate is unable to meet growing demands of pharmaceutical industries. The present study is aimed to find a highly effective and a rapid method for the Micropropagation of this medicinal plant to meet the demand. The explants of V. negundo were cultured on Murashige and Skoogs (MS) medium supplemented with different concentrations of auxins, cytokinins and sucrose. Among different combinations used, Murashige and Skoog medium with 0.5 (mg L–1) 6-benzylaminopurine + 0.5 (mg L–1) α-naphthalene acetic acid, containing 0.8% agar and 25% sugarcane juice exhibited highest shoot induction (80%) with highest number of shoots per explant (3 - 4) supplemented with 3% Sucrose and 0.8% (w/v) agar within 20 days. |
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| Keywords | ||
| Vitex Negundo, Benzyl amino purine, Naphthalene acetic acid, and proliferation | ||
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