Characterisation of alkaline protease from newly isolated Bacillus strain NBB 04

Bacterial isolate NBB04 producing alkaline protease was obtained from the waste of the paper industry. Gram Positive strain shows avtivity in alkalime medium with maximum activity abtained after 48 hours of incubation. The crude enzyme shows optimum temperature of 40oC and oprimum pH of 10.0. The enzyme has stability of 24 hours at pH 10 at temperature of 40 and 50oC. in the temperature range of 37-45oC the temperature remain 100% stable thereby can be potential candidate for various application of this enzyme in various industries involving protease.