Antioxidant And Polyphenol Activity of Alkaliphilic Isolates

Extremophiles can produce antioxidants and polyphenols in order to keep the cell protected from oxidants and extreme conditions. In order to test this hypothesis the samples were taken from the alkaline sites. Various bacterial isolates were isolated. These organisms were grown in the alkaline broth medium. Study of antioxidant and polyphenol activity was performed from the cell free extracts. The results were not consistent hence method was modified. Cell free extracts were dried for few days and then antioxidant and polyphenol activity of the extracts were detected. But these cell free extracts didn’t show antioxidant and polyphenol activity. It is also possible that the bacteria can accumulate these antioxidants inside the cell. Hence intracellular extracts of the bacteria were prepared by lysing the cells with the glass beads and extraction with methanol. These intracellular extracts were dried at room temperature. The antioxidant and polyphenol activities were detected.