Diazinon induced changes in blood biochemistry of Channa striatus

The acute effect of diazinon on the Channa striatus (Bloch) was assessed by comparing the biochemical blood plasma profiles of a control group and a group exposed to the effect of the pesticide DiazintolR. The fishes were exposed to sublethal concentration of diazinon and control for 96 h. Fish of particular size (22-24 cm) and weight (25-30 g) range were used irrespective of their sex for the experiments. The test solutions were renewed every 24 hr to maintain the optimum dissolved oxygen level. To evaluate the acute toxicity studies the static renewal toxicity test were conducted according to the methods recommended by American Public Health Association (1960). In the present investigation the median lethal concentration (LC50) of diazinon for 24, 48, 72 and 96 hr were analyzed. The toxicant concentration used in the present series of tests were approximately the wide range of concentrations viz., Control, LC10, LC20, LC30, LC40, LC50, LC60, LC70, LC80, LC90, LC99 aqueous solutions were prepared. Each group twenty one fishes were introduced with three replications. The blood samples from the challenged fishes were taken after every 20, 40 and 60 minute in fishes exposed to diazinon. The challenged fishes showed a significant rise in A:G ratio, creatinine, urea, uric acid, glucose, cortisol, bilrubin and cholesterol. However, opposite trend was observed in protein, albumin, globulin, liver glycogen, serum lipid and serum phosphorous.